Journal: Cancer cell international

Article Title: ADAM8 is expressed widely in breast cancer and predicts poor outcome in hormone receptor positive, HER-2 negative patients.

doi: 10.1186/s12935-023-03024-3

Figure Lengend Snippet: Fig. 3 ADAM8 is expressed in all breast cancer subtypes. Freshly cut consecutive TMA slides containing breast cancer patient primary tumor samples were subjected to IHC with ADP2 vs isotype-matched control IgG2b at 1:50 dilutions. H&E staining was used to confirm tissue quality and exclude depleted samples. A Representative images of ADP2-stained TNBC samples with increasing ADAM8 expression and associated H-scores are shown. Staining with IgG2b was negative as expected (not shown). Upper panels: whole core images, scale bar: 200 μm; lower panels: magnified images of same samples, scale bar: 50 μm. B Representative images of ADP2 IHC staining for ADAM8 in breast cancer patient samples of non-TNBC HR−/HER2+, HR+/HER2− and HR+/HER2+ subtypes. Specimens with high H-scores (e.g., 250–300) are shown. Upper panels: whole core images, scale bar: 200 μm; lower panels: magnified images of same samples, scale bar: 50 μm

Article Snippet: Stable clones of HEK-293 cells expressing human ADAM8 (HEK-A8) or control empty vector DNA (HEK-EV) were prepared by transfection of 1 μg of either pCMV6human ADAM8 Variant 1-AC-GFP plasmid (RG213386, Origene, Rockville, MD, USA) or control pCMV6-ACGFP DNA (PS100010, Origene, Rockville, MD, USA), respectively, using Lipofectoamine 2000 (11668019, Invitrogen, Waltham, MA), followed by selection in 500 μg/ ml G418.

Techniques: Control, Staining, Expressing, Immunohistochemistry

Journal: Nucleic Acids Research

Article Title: Determination of local chromatin interactions using a combined CRISPR and peroxidase APEX2 system

doi: 10.1093/nar/gkz134

Figure Lengend Snippet: Combining CRISPR system with peroxidase APEX2 to identify local chromatin interactions. ( A ) Schematic of CAPLOCUS system. ( B ) The three components of the CAPLOCUS system: TO-NLS_dCas9_NLS, the RNA binding partner fused with peroxidase APEX2_NLS, and target-specific sgRNA.

Article Snippet: To create the MS2-APEX2_NLS fusion protein expression vector, APEX2 was amplified by polymerase chain reaction (PCR) from pcDNA3 Connexin43-GFP-APEX2 (Addgene plasmid: 49385) and cloned into the pHAGE-EFS-MCP-3XBFPnls vector (Addgene plasmid: 75384) with BamHI and XhoI.

Techniques: CRISPR, RNA Binding Assay